Bcl-2 (phospho Ser70) rabbit pAb

• Catalog Number: orb764147
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Bcl-2 (phospho-Ser70)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Monkey
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human BCL-2 around the phosphorylation site of Ser70. AA range:41-90
• MW: 26
• Tested applications: ELISA, IHC-P, IP, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-BCL2 antibody, anti-Apoptosis regulator Bcl-2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Phospho-Bcl-2 (S70) Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of HeLa cells using Phospho-Bcl-2 (S70) Polyclonal Antibody diluted at 1:1000.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BCL-2 (Phospho-Ser70) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with LPS (40nM, 30mins), K562 cells treated with calyculin A (50ng/ml, 30mins) and COS-7 cells treated with H2O2 (1ng/ml, 15mins), using BCL-2 (Phospho-Ser70) Antibody. The lane on the right is blocked with the phospho peptide.