AurB/C rabbit pAb

• Catalog Number: orb764610
• Category: Antibodies
• Description: Rabbit polyclonal antibody to AurB/C
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human AurB/C. AA range:201-250
• MW: 35+39
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-AURKB antibody, anti-AIK2 antibody, anti-AIM1
• Note: For research use only

Western Blot analysis of various cells using AurB/C Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Western blot analysis of lysates from 293, HeLa, and fHepG2 cells, treated with Paclitaxel 1uM 24h, using AurB/C Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from COLO205 cells using AurB/C antibody.

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).