ATF-2 (phospho Thr71) rabbit pAb

• Catalog Number: orb764142
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ATF-2 (phospho-Thr71)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human ATF2 around the phosphorylation site of Thr71 or 53. AA range:38-87
• MW: 52
• Tested applications: ELISA, IHC-P, IP, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-ATF2 antibody, anti-CREB2 antibody, anti-CREB
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Phospho-ATF-2 (T71) Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of HuvEc cells using Phospho-ATF-2 (T71) Polyclonal Antibody diluted at 1:1000.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ATF2 (Phospho-Thr71 or 53) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with UV, using ATF2 (Phospho-Thr71 or 53) Antibody. The lane on the right is blocked with the phospho peptide.