ATF-1 rabbit pAb

• Catalog Number: orb764595
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ATF-1
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human ATF1. AA range:176-225
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 36
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-ATF1 antibody, anti-Cyclic AMP-dependent tran
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using ATF-1 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Western blot analysis of 293T 3T3 HELA KB SH-SY5Y lysis using ATF-1 antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunofluorescence analysis of HUVEC cells, using ATF1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HepG2, COLO205, and HeLa cells, using ATF1 Antibody. The lane on the right is blocked with the synthesized peptide.