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Catalog Number | orb1255948 |
---|---|
Category | Antibodies |
Description | ARFGAP3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 217-516 of human ARFGAP3 (NP_055385.3). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 57kDa |
Target | ARFGAP3 |
UniProt ID | Q9NP61 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | ARFGAP3, ADP-ribosylation factor GTPase activating Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using ARFGAP3 antibody (orb1255948) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse stomach using ARFGAP3 antibody (orb1255948) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using ARFGAP3 antibody (orb1255948) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using ARFGAP3 antibody (orb1255948) at dilution of 1:100 (40x lens).
ELISA, WB | |
Bovine, Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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