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Item 1 of 3
Apolipoprotein A I Antibody / APOA1
Catalog Number: orb699510
| Catalog Number | orb699510 |
|---|---|
| Category | Antibodies |
| Description | Apolipoproteins are protein components of plasma lipoproteins. The human apoA-I gene encodes a single chain, 243 amino acid protein which promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. It can function as a cofactor for lecithin cholesterolacyltransferase (LCAT), which is responsible for the formation of most plasma cholesteryl esters. The human apoA-II gene encodes the second most abundant protein of HDL particles, where it influences plasma levels of free fatty acids (FFA). The human apoA-IV gene encodes a 396 amino acid preprotein, which after proteolytic processing is secreted from the intestine in association with chylomicron particles. ApoA-IV is a potent activator of LCAT in vitro. The human apoA-V gene encodes a 366 amino acid protein that is believed to be an important determinant of plasma triglyceride levels. |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | Mouse IgG1, kappa |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Immunogen | A portion of amino acids 83-167 from the human protein was used as the immunogen for the APOA1 antibody. |
| UniProt ID | P02647 |
| Tested applications | IHC-P |
| Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT |
| Application notes | Optimal dilution of the APOA1 antibody should be determined by the researcher. |
| Antibody Type | Primary Antibody |
| Clone Number | APOA1/3661 |
| Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Hazard Information | This APOA1 antibody is available for research use only. |
| Note | For research use only |
IHC staining of FFPE human liver with APOA1 antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver with APOA1 antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free APOA1 antibody as confirmation of integrity and purity.
