CD235a Antibody-PE labled

SKU: orb1293736

Description

The CD235a Antibody-PE labled is suitable for FC, IF. It is a Monoclonal, PE conjugated antibody. Purification: The antibody was purified by affinity chromatography.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: FC, IF
• Dilution range: 10 μl / assay
• Reactivity: Human
• Application Notes:
  1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Clone No.: JC159
• Purification: The antibody was purified by affinity chromatography.
• Conjugation: PE

Storage & Handling

• Storage: Shipped and store at 4°C for one year. Do not freeze.
• Form/Appearance: Mouse IgG1. Liquid in PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
• Disclaimer: For research use only

Alternative Names

GPA; Glycophorin-A; MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; CD235a

Quick Database Links

UniProt

P02724

NCBI Gene ID

2993