Akt1/3 rabbit pAb

SKU: orb768039

Description

Rabbit polyclonal antibody to Akt1/3

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human AKT1. AA range:406-455
• Molecular Weight: 56
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-AKT1 antibody, anti-PKB antibody, anti-RAC antibody, anti-RAC-alpha serine/threonine-protein kinase antibody, anti-Protein kinase B antibody, anti-PKB antibody, anti-Protein kinase B alpha antibody, anti-PKB alpha antibody, anti-Proto-oncogene c-Akt antibody, anti-RAC-PK-alpha antibody, anti-AKT3 antibody, anti-PKBG antibody, anti-RAC-gamma serine/threonine-protein antibody

Western Blot analysis of 3T3 cells using Akt1/3 Polyclonal Antibody diluted at 1:1000.

Western blot analysis of lysates from A549 cells, using AKT1/3 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).