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| Catalog Number | orb764490 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to AIF-M1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AIFM1. AA range:51-100 |
| MW | 67 |
| Tested applications | ELISA, IF, IHC-P, WB |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-AIFM1 antibody, anti-AIF antibody, anti-PDCD8 Read more... |
| Note | For research use only |
Western Blot analysis of various cells using AIF-M1 Polyclonal Antibody diluted at 1:1000.
Western Blot analysis of Jurkat cells using AIF-M1 Polyclonal Antibody diluted at 1:1000.
Western blot analysis of lysates from HUVEC cells, HepG2 cells, HeLa cells, and Jurkat cells, using AIFM1 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
