AGA Antibody

SKU: orb1254863

Description

AGA Antibody

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:1000IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-346 of human AGA (NP_000018.2).
• Target: AGA
• Molecular Weight: Observed: 18kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

AGU, ASRG, GA, N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase, N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase, aspartylglucosylamine deaspartylase, glycosylasparaginase

Western blot analysis of extracts of various cell lines, using AGA antibody (orb1254863) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of HeLa cells using AGA antibody (orb1254863). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

AGA

UniProt

P20933