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Item 1 of 4
Item 1 of 4
| Catalog Number | orb769496 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to 14-3-3 Theta/Tau (phospho-Ser232) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human 14-3-3 thet/tau around the phosphorylation site of Ser232. AA range:196-245 |
| MW | 28 |
| Tested applications | ELISA, IF, IHC-P, WB |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-YWHAQ antibody, anti-14-3-3 protein theta ant Read more... |
| Note | For research use only |
Immunofluorescence analysis of Hela cell. 1, 14-3-3 θ/τ (phospho Ser232) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min).
Western blot analysis of 293T MOUSE-BRAIN using p-14-3-3 θ/τ (S232) antibody. Antibody was diluted at 1:500.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using 14-3-3 thet/tau (Phospho-Ser232) Antibody.
Immunofluorescence analysis of HeLa cells, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is blocked with the phospho peptide.
