Z-IETD-AFC

SKU: orb1693832

Description

Z-IETD-AFC

Key Properties

• CAS Number: 219138-02-0
• MW: 821.75
• Purity: 98.67%
• Formula: C₃₇H₄₂F₃N₅O₁₃
• SMILES: CC[C@H](C)[C@H](NC(=O)OCc1ccccc1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(=O)Nc1ccc2c(cc(=O)oc2c1)C(F)(F)F
• Target: Others
• Solubility: DMSO:4 mg/mL (4.87 mM);H2O:4 mg/mL (4.87 mM)

Bioactivity

• Cell Research:
  Instructions a. Solution preparation: 1. Prepare Z-IETD-AFC powder, dissolve it in DMSO, prepare a 10 mM stock solution, store below - 15°C, and protect from light. Prepare DTT (1 M), EDTA (100 mM), and Tris buffer (20 mM, pH=7.4). 2. Mix 50 μL Z-IETD-AFC stock solution, 100 μL DTT, 400 μL EDTA, and 10 mL Tris buffer to prepare a 2x Caspase substrate detection solution, in which the final concentration of Z-IETD-AFC is 50 μM. b. Operation steps: 1. Sample processing: Mix samples or cell lysates containing Caspase-8 activity with an equal volume of 2x Caspase substrate detection solution and incubate at room temperature for at least 1 hour. 2. Detection and analysis: The fluorescence intensity was monitored using a fluorescence microplate reader with an excitation wavelength of 376 nm and an emission wavelength of 482 nm. The activity of Caspase-8 was quantified based on the changes in fluorescence intensity.

Storage & Handling

• Storage: store at low temperature,keep away from moisture,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ZIETDAFC, Z-IETD-AFC, Z IETD AFC

Compound Identifiers

PubChem CID

71312079