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| Catalog Number | orb770692 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to PRAK (phospho-Thr182) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MAPKAPK5 around the phosphorylation site of Thr182. AA range:148-197 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 60 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-MAPKAPK5 antibody, anti-PRAK antibody, anti-M Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MAPKAPK5 (Phospho-Thr182) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3nM 40', using MAPKAPK5 (Phospho-Thr182) Antibody. The lane on the right is blocked with the phospho peptide.
