PRAK (phospho Thr182) rabbit pAb

• Catalog Number: orb770692
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PRAK (phospho-Thr182)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human MAPKAPK5 around the phosphorylation site of Thr182. AA range:148-197
• MW: 60
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-MAPKAPK5 antibody, anti-PRAK antibody, anti-M
• Note: For research use only

Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MAPKAPK5 (Phospho-Thr182) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3nM 40', using MAPKAPK5 (Phospho-Thr182) Antibody. The lane on the right is blocked with the phospho peptide.