Nucleolin Antibody

• Catalog Number: orb864252
• Category: Antibodies
• Description: Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein A affinity chromatography
• Immunogen: Recombinant full length human protein was used as the immunogen for the Nucleolin antibody.
• UniProt ID: P19338
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the Nucleolin antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: NCL/7014R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This Nucleolin antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human salivary gland with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human kidney tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human kidney tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative: IHC staining of FFPE mouse stomach tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative: IHC staining of FFPE cat liver tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative: IHC staining of FFPE horse lung tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative: IHC staining of FFPE cow heart tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Jurkat cell lysate with Nucleolin antibody (clone NCL/7014R). Predicted molecular weight ~77 kDa, can be observed at 100~110 kDa.

SDS-PAGE analysis of purified, BSA-free Nucleolin antibody (clone NCL/7014R) as confirmation of integrity and purity.