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Nucleolin Antibody

Catalog Number: orb864252

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb864252
CategoryAntibodies
DescriptionRecognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells.
Species/HostRabbit
ClonalityRecombinant
Clone NumberNCL/7014R
Tested applicationsFACS, IF, IHC-P, WB
ReactivityHuman
IsotypeRabbit IgG, kappa
ImmunogenRecombinant full length human protein was used as the immunogen for the Nucleolin antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein A affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis Nucleolin antibody is available for research use only.
UniProt IDP19338
StorageStore the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the Nucleolin antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
Nucleolin Antibody

IHC staining of FFPE human salivary gland with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Nucleolin Antibody

IHC staining of FFPE human kidney tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Nucleolin Antibody

IHC staining of FFPE human kidney tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Nucleolin Antibody

Negative: IHC staining of FFPE mouse stomach tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Nucleolin Antibody

Negative: IHC staining of FFPE cat liver tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Nucleolin Antibody

Negative: IHC staining of FFPE horse lung tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Nucleolin Antibody

Negative: IHC staining of FFPE cow heart tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Nucleolin Antibody

Western blot testing of human Jurkat cell lysate with Nucleolin antibody (clone NCL/7014R). Predicted molecular weight ~77 kDa, can be observed at 100~110 kDa.

Nucleolin Antibody

SDS-PAGE analysis of purified, BSA-free Nucleolin antibody (clone NCL/7014R) as confirmation of integrity and purity.

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