VEGFA Rabbit Polyclonal Antibody

• Catalog Number: orb500818
• Category: Antibodies
• Description: VEGFA Rabbit Polyclonal Antibody
• Target: VEGFA
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human VEGF (27-120/232aa)
• UniProt ID: P15692
• MW: 24 kDa
• Tested applications: WB
• Dilution range: WB=1:500-1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Vascuoar endothelial growth factor A; VEGF A; vasc
• Research Area: Angiogenesis, Angiogenesis and Vasculogenesis, Ang
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Heart (Mouse) Lysate at 30 ug, Primary: Anti-VEGF (orb500818) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 25 kD.

Cell: Hela, Concentration: 1:100, Host/Isotype: Rabbit/IgG, Flow cytometric analysis of Rabbit antibody (orb500818) on Hela (green) compared with isotype control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody.

Sample: heart (Mouse) Lysate at 40 ug, A549 Cell (Human) Lysate at 30 ug, Primary: Anti-VEGF (orb500818) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 24 kD.

Sample: Kidney (Mouse) Lysate at 30 ug, Primary: Anti-VEGF (orb500818) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 25 kD.