SOX10 Antibody

SKU: orb1252142

Description

SOX10 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 µg/mLWestern blot: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the SOX10 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: SOX10/991
• Immunogen: Amino acids 115-269 of the human protein were used as the immunogen for the SOX10 antibody.
• Target: SOX10
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SOX10 Antibody: SRY (sex determining region Y)-box 10, DOM,WS4, PCWH, WS2E, WS4C

Western blot testing of A) partial recombinant protein B) A375 cell lysate using SOX10 antibody (SOX10/991). Predicted molecular weight ~50 kDa.

IHC testing of FFPE human melanoma with SOX10 antibody (clone SOX10/991). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Analysis of HuProt(TM) microarray containing more than 19000 full-length human proteins using SOX10 antibody (clone SOX10/991). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE mouse brain with SOX10 antibody (clone SOX10/991). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free SOX10 Antibody (clone SOX10/991). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

SOX10

UniProt

P56693