SOST Antibody (N-term)

SKU: orb36419

Description

Purified rabbit polyclonal antibody.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: IHC-P: 1:10-50, WB: 1:1000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD34500
• Immunogen: Synthetic Peptide
• Target: SOST
• Molecular Weight: 24031
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Sclerostin, SOST

Anti-SOST Antibody (N-term) at 1:2000 dilution + Recombinant human SOST protein lysate. Lysates/proteins at 20 ng per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 24 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from mouse liver, mouse lung, rat kidney tissue lysate (from left to right), using SOST Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

All lanes: Anti-SOST Antibody (N-term) at 1:1000 dilution. Lane 1: human kidney lysates. Lane 2: human liver lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 24 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-SOST Antibody (N-term) at 1:2000 dilution. Lane 1: mouse lung lysates. Lane 2: human liver lysates. Lane 3: Hela whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 24 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

SOST

UniProt

Q9BQB4

RefSeq (Protein):NP_079513.1

Ye, Weiyuan et al. Computational and functional characterization of four SNPs in the SOST locus associated with osteoporosis Bone, 108, 132-144 (2018)

PubMed: 29307778