SLC9A3R1 Antibody (Center)

SKU: orb1930328

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Infectious Disease & Virology, Signal Transduction

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB - 1:2000, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD43038
• Immunogen: This SLC9A3R1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids of human SLC9A3R1. Antigen Region: 168-197 aa.
• Target: NHERF1 (HGNC:11075)
• Molecular Weight: 38868 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Na(+)/H(+) exchange regulatory cofactor NHE-RF1, NHERF-1, Ezrin-radixin-moesin-binding phosphoprotein 50, EBP50, Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1, SLC9A3R1, NHERF, NHERF1

SLC9A3R1 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

SLC9A3R1 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35 ug/lane). This demonstrates the SLC9A3R1 antibody detected the SLC9A3R1 protein (arrow).

All lanes: Anti-SLC9A3R1 Antibody (Center) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: T47D whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 39 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

NHERF1 (HGNC:11075)

UniProt

O14745

RefSeq (Protein):NP_004243.1