Sheep IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

SKU: orb347933

Description

Sheep IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:1,000 - 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:400 - 1:1,600
• Reactivity: Sheep
• Application Notes:
  Secondary antibody conjugated to alkaline phosphatase is available in a variety of formats. Conjugated Secondary Antibody is suitable for western blot, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Sheep IgG whole molecule
• Purity: Secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins.
• Conjugation: AP

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.09% (w/v) Sodium Azide
• Concentration: 0.5 mg/mL
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-Sheep IgG alkaline phosphatase Conjugated Antibody, Rabbit Anti-Sheep IgG Antibody alk phos Conjugation