SC79

SKU: orb1305828

Description

SC79 is a blood-brain barrier permeable small molecule that acts as a specific AKT agonist by binding to its PH domain. It activates cytoplasmic AKT while inhibiting its membrane translocation, demonstrating neuroprotective effects in both cellular and animal models of neurological injury.

Research Area

Signal Transduction

Key Properties

• CAS Number: 305834-79-1
• MW: 364.78
• Purity: >99.99% (May vary between batches)
• Formula: C₁₇H₁₇ClN₂O₅
• SMILES: CCOC(=O)C(C#N)C1C(C(=O)OCC)=C(N)Oc2ccc(Cl)cc12
• Target: Akt
• Solubility: DMSO:242.5 mg/mL (664.78 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5.5 mg/mL (15.08 mM)

Bioactivity

• Target IC50:
  Akt:4 µg/mL
• In Vivo:
  METHODS: To detect in vivo activity, SC79 (0.04 mg/g) was injected intraperitoneally into a C57 Black/6 mouse model of copper relaxation demyelination, and 5 min later middle cerebral artery occlusion (MCAO) was performed to construct an ischemic stroke model. RESULTS: A single dose of SC79 reduced the size of neocortical lesions by more than 35% and 40% at 24 h after MCAO and 1 week after MCAO, respectively. METHODS: To investigate the effect on liver injury, SC79 (10 mg/kg) was injected intraperitoneally into C57BL/6 mice, and d-Gal/LPS was injected 0.5 h later to induce liver injury. RESULTS: SC79 protected mice from TNF-α-mediated liver injury induced by d-Gal/LPS.
• In Vitro:
  METHODS: Human cervical cancer cells were starved of HeLa serum for 1 h, treated with SC79 (4 µg/mL) for 30 min, and the expression levels of target proteins were detected by Western Blot. RESULTS: SC79 enhanced AKT phosphorylation, and SC79-induced AKT phosphorylation mainly occurred in the cytoplasm. METHODS: Human lung cancer cells A549 were treated with SC79 (10 µg/mL) for 24 h. The gene expression level was detected by qPCR. RESULTS: SC79 treatment up-regulated the expression of Nrf-2 (NFE2L2) gene itself as well as the downstream targets HO-1 and NQO-1.
• Cell Research:
  HsSultan or NB4 cells (2.5 × 105) are plated in a 24-well plate in 500 μL of phenol red-free RPMI medium supplemented with 10% FBS. After incubation for 24 hours, each compound (8 μg/mL) is added and cultured for overnight (16–20 h). Fifty microliters of MTT solution (5 mg/mL in PBS) are added to each well. Following 2 hrs incubation, the purple formazan crystals are dissolved by directly adding in 500 μL of isopropanol with 0.1 M HCl to each well. After clearing the cell debris by centrifugation, the absorbance is measured at a wavelength of 570 nm.(Only for Reference)

Storage & Handling

• Storage: store at low temperature,keep away from moisture | Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Akt, Inhibitor, inhibit, SC 79, SC79, SC-79, PKB, Protein kinase B

Compound Identifiers

PubChem CID

2810830