SC-79

SKU: orb1225359

Description

A unique specific Akt activator; binds to the PH domain of Akt and inhibits Akt membrane translocation, but paradoxically activates Akt in the cytosol; induces the p-Akt (Ser473) expression and significantly increases the expression of Bcl-2 and p-GSK-3β (Ser9), decreases the protein levels of Bax, cytoplasm cytochrome C, and cleaved caspase-3.; augments neuronal survival in mouse model for ischemic strok.

Key Properties

• CAS Number: 305834-79-1
• MW: 364.7803
• Purity: >98% (HPLC)
• Formula: C₁₇H₁₇ClN₂O₅
• SMILES: NC1=C(C(OCC)=O)C(C(C(OCC)=O)C#N)C2=CC(Cl)=CC=C2O1
• Target: Akt
• Solubility: 10 mM in DMSO

Bioactivity

• In Vivo:
  Animal model: Male, age-matched (6-to 8-weekeold) C57BL/6 or BALB/c mice weighing 16 to 18 g. Dosage: 10 mg/kg. Administration: Intraperitoneally at 0.5 hour before the i.p. administration of an agonistic anti-Fas Jo2 antibody at a lethal dose of 0.5 and 0.4 mg/kg for C57BL/6 and BALB/c mice, respectively. Result: Treatment of mice with 10 mg/kg of SC79 at 0.5 hour before Jo2 injection increased mouse survival at 12 hours after Jo2 injection from 0% to 35%, and no additional mortality was observed to the end of the 2-month observation period. Animal model: Male, age-matched (6 to 8 weeks old) C57BL/6 mice weighing 16-18 g. Dosage: 10 mg/kg. Administration: Intraperitoneally at 0.5 h before i.p. administration of 400 mg/kg of D-galactosamine (D-Gal) and 60 μg/kg of lipopolysaccharide (LPS) for C57BL/6 mice. Result: Gal/LPS challenge there was more bleeding on the liver of the vehicle control-treated mice as compared to that of SC79-treated mice. A single dose of SC79 significantly reduced Gal/LPS-mediated liver damage but not an infiltration of inflammatory cells in liver sections.
• In Vitro:
  Western blot analysis. Cell line: HeLa cells. Concentration: 4 μg/mL (10.96 μM). Incubation time: 30 min. Result: Induced cytosolic phosphorylation of Akt.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SC79

Quick Database Links

Gene Symbol

Akt