SARS-CoV-2 (COVID-19) S1 Protein, His Tag

Catalog Number: orb638775

Catalog Number	orb638775
Category	Proteins
Description	It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Reactivity	Virus
Tag	C-10×His
Form/Appearance	Powder
Purity	95%
Conjugation	Unconjugated
MW	76.9 kDa
Target	Spike S1
Protein Sequence	QHD43416.1
Expression System	HEK293
Biological Origin	SARS-CoV-2
Expression Region	Val 16 - Arg 685
Endotoxins	1.0 EU per μg
NCBI	QHD43416.1
Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Buffer/Preservatives	PBS, pH 7.4
Note	For research use only
Application notes	This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 76.9 kDa. The protein migrates as 100-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expiration Date	6 months from date of receipt.

Item 1 of 1

Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 33.7 kDa after removal of the signal peptide. The apparent molecular mass of S1-NTD-His is approximately 55 kDa due to glycosylation.
Source:	Mammalian

Sizes Available: 10 μg, 50 μg, 100 μg

Applications:	ELISA, SDS-PAGE, WB
Purity:	> 90% as determined by Bis-Tris PAGE
MW:	120 kD
Source:	HEK293 cells

Purity:	Greater than 95.0% as determined by SDS-PAGE.
Source:	HEK293 Cells