S100B (Astrocyte and Melanoma Marker) Antibody

• Catalog Number: orb1410351
• Category: Antibodies
• Description: Mouse monoclonal antibody to S100B (Astrocyte and Melanoma Marker)
• Target: S100B
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b
• Conjugation: Unconjugated
• Reactivity: Bovine, Human, Mouse, Rat
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Purification: Protein A
• Immunogen: Recombinant full-length human S100B protein
• UniProt ID: P04271
• MW: 10-12kDa
• Tested applications: IHC
• Clone Number: S100B/4153
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NEF; S-100 protein beta chain; S100 calcium bindin
• Research Area: Cardiovascular Research, Cell Biology, Immunology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Analysis of Protein Array containing more than 19000 full-length human proteins using S100B-Monospecific Mouse Monoclonal Antibody (S100B/4153). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human brain stained with S100B Mouse Monoclonal Antibody (S100B/4153) at 2 ug/ml. HIER: Tris/EDTA, pH9.0, 45 min. 2°C: HRP-polymer, 30 min. DAB, 5 min.

Formalin-fixed, paraffin-embedded human brain stained with S100B Mouse Monoclonal Antibody (S100B/4153) at 2 ug/ml. Inset: PBS instead of primary antibody, secondary negative control.

SDS-PAGE Analysis S100B Mouse Monoclonal Antibody (S100B/4153). Confirmation of Integrity and Purity of Antibody.