RecombinantMIP-1β/CCL4,Human

• Catalog Number: orb1494792
• Category: Proteins
• Description: Macrophage inflammatory protein 1 beta (MIP-1β), also known as Chemokine (C-C motif) ligand 4(CCL4), is a small cytokine belonging to the CC chemokine family. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. MIP-1βis a major HIV-suppressive factor produced by CD8+ T cells. Perforin-low memory CD8+ T cells are the most common T-cells that normally synthesize MIP-1-beta in humans. MIP-1βhas been shown to interact with CCL3. It can signal through the CCR5 receptor.Recombinant MIP-1 beta/CCL4 produced in E.coli is a single non-glycosylated polypeptide chain containing 69 amino acids. A fully biologically active molecule, rhMIP-1 beta/CCL4 has a molecular mass of 7.6 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Purity: > 95% as analyzed by SDS-PAGE and HPLC.
• Purification: > 95% as analyzed by SDS-PAGE and HPLC.
• Protein Sequence: APM GSDPPTACCF SYTARKLPRN FVVDYYETSS LCSQPAVVFQ TKRSKQVCAD PSESWVQEYV YDLELN
• MW: 7.6 kDa, observed by reducing SDS-PAGE.
• Application notes: Reconstituted in ddH2O or PBS at 100 μg/ml.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Source: Escherichia coli.
• Biological Activity: The EC50 value of human MIP-1 beta /CCL4 on Caˆ2+ mobilization assay in CHO-K1/ Gα15/hCCR5 cells (human Gα15 and human CCR5 stably expressed in CHO-K1 cells) is less than 100 ng/ml.
• Storage: Lyophilized recombinant human MIP-1 beta /CCL4 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, human MIP-1 beta /CCL4 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
• Alternative names: MIP1β, Macrophage Inflammatory Protein-1β, CCL4, A
• Note: For research use only
• Expiration Date: 6 months from date of receipt.