RecombinantMCP‑3/CCL7,Human(CHO-expressed)

SKU: orb1494687

Description

Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine that was previously called monocyte-specific chemokine 3 (MCP-3). Due to CCL7 possessing two adjacent N-terminal cysteine residues in its mature form, it is classified within the subfamily of chemokines known as CC chemokines. CCL7 specifically attracts monocytes, and regulates macrophage function. It is produced by certain tumor cell lines and by macrophages. This chemokine is located on chromosome 17 in humans, within a large cluster containing many other CC chemokines and is most closely related to CCL2. CCL7 can signal through the CCR1, CCR2 and CCR3 receptors.Recombinant human MCP-3/CCL7 produced in CHO cells is a polypeptide chain containing 76 amino acids. A fully biologically active molecule, rhMCP‑3/CCL7 has a molecular mass of 10-18 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Images & Validation

• Application Notes:
  Reconstituted in ddH2O or PBS at 100 μg/ml.

Key Properties

• Source: CHO
• Biological Activity: The EC50 value of human MCP‑3/CCL7 on Caˆ2+ mobilization assay in CHO-K1/ Gα15/hCCR1 cells (human Gα15 and human CCR1 stably expressed in CHO-K1 cells) is less than 1.5 μg/ml.
• Molecular Weight: 10-18 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: QPVGINTSTTCCYRFINKKIPKQRLESYRRTTSSHCPREAVIFKTKLDKEICADPTQKWVQDFMKHLDKKTQTPKL
• Purification: > 98% as analyzed by SDS-PAGE.
• Purity: > 98% as analyzed by SDS-PAGE.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.

Storage & Handling

• Storage: Lyophilized recombinant human MCP-3°CCL7 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, human MCP-3°CCL7 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Small inducible cytokine A7, CCL7, Monocyte chemotactic protein 3, MCP3, Monocyte chemoattractant protein 3, MARC