RecombinantMCP-5/CCL12,Mouse

SKU: orb1494685

Description

Monocyte Chemotactic Protein-5(MCP-5), also known as Chemokine (C-C motif) ligand 12(CCL12), is a small cytokine belonging to the CC chemokine family that has been characterized in mice. Due to its similarity with human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. MCP-5 specifically attracts eosinophils, monocytes and lymphocytes.It can signal through the CCR2 receptor.Recombinant Mouse MCP-5/CCL1 produced in CHO cells is a polypeptide chain containing 82 amino acids. A fully biologically active molecule, rmMCP-5/CCL12 has a molecular mass of 11 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Images & Validation

• Application Notes:
  Reconstituted in ddH2O or PBS at 100 μg/ml.

Key Properties

• Source: CHO
• Biological Activity: The EC50 value of mouse MCP-5/CCL12 on Caˆ2+ mobilization assay in CHO-K1/G15/mCCR2 cells (human G15 and mouse CCR2 stably expressed in CHO-K1 cells) is less than 0.5 μg/ml.
• Molecular Weight: 9.3-11 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWV KNSINHLDKTSQTFILEPSCLG
• Purification: > 98% as analyzed by SDS-PAGE.
• Purity: > 98% as analyzed by SDS-PAGE.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.

Storage & Handling

• Storage: Lyophilized recombinant Mouse MCP-5°CCL12 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse MCP-5°CCL12 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

MCP-5,Monocyte Chemotactic Protein-5, CCL12