Rat VEGF 164

• Catalog Number: orb2994452
• Category: Proteins
• Description: Recombinant Rat Vascular Endothelial Growth Factor A is produced by our Yeast expression system and the target gene encoding Ala27-Arg190(Ala36Thr) is expressed.
• Conjugation: Unconjugated
• Tag: Tag free
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• UniProt ID: P16612
• MW: Predicted: 19.2 KDa. Observed: 18-23 KDa, reducing conditions
• Expression System: P.Pichia
• Endotoxins: < 0.1 EU/µg as determined by LAL test.
• Biological Origin: Rat
• Biological Activity: ELISA: Immobilized Rat VEGF 164(Cat#orb2994452) at 2μg/ml (100 μl/well) can bind Mouse VEGFR2-Fc(Cat#orb2993886).The ED50 of Mouse VEGFR2-Fc(Cat#orb2993886) is 22.17 ng/ml (Regularly tested).. BLI: Loaded Mouse VEGFR2-Fc(Cat#orb2993886) on Protein A Biosensor, can bind Rat VEGF 164(Cat#orb2994452) with an affinity constant of 0.26 nM as determined in BLI assay. Loaded Human VEGF Antibody (5E7)-Fc(Cat#DA082) on AHC Biosensor, can bind Rat VEGF 164(Cat#orb2994452) with an affinity constant of 1.09 nM as determined in BLI assay.
• Expression Region: Ala27-Arg190(Ala36Thr)
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Alternative names: Vascular endothelial growth factor A; Vascular per
• Note: For research use only
• Expiration Date: 6 months from date of receipt.