Mouse VEGF 164

• Catalog Number: orb2993968
• Category: Proteins
• Description: Recombinant Mouse Vascular Endothelial Growth Factor A is produced by our Yeast expression system and the target gene encoding Ala27-Arg190 is expressed.
• Conjugation: Unconjugated
• Tag: Tag free
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Purity: SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
• UniProt ID: Q00731
• MW: Predicted: 19.27 KDa. Observed: 18-22 KDa, reducing conditions
• Expression System: P.Pichia
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Biological Origin: Mouse
• Biological Activity: BLI: Loaded Mouse VEGFR2-Fc(Cat#orb2993886) on Protein A Biosensor, can bind Mouse VEGF 164(Cat#orb2993968) with an affinity constant of 0.44 nM as determined in BLI assay. Loaded Human VEGF Antibody (5E7)-Fc(Cat#DA082) on AHC Biosensor, can bind Mouse VEGF164(Cat#orb2993968) with an affinity constant of 3.63 nM as determined in BLI assay. Loaded Human VEGF-165 Antibody (6D9)-Fc(Cat#DA083) on AHC Biosensor, can bind Mouse VEGF164(Cat#orb2993968) with an affinity constant of 1.07 nM as determined in BLI assay.
• Expression Region: Ala27-Arg190
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Alternative names: Vascular endothelial growth factor A; VEGF-A; Vasc
• Note: For research use only
• Expiration Date: 6 months from date of receipt.