Recombinant Mouse CD40L Protein

SKU: orb2994091

Description

Recombinant Mouse CD40 Ligand is produced by our Mammalian expression system and the target gene encoding Met112-Leu260 is expressed with a 6His tag at the N-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Mouse
• Biological Activity: ELISA: Immobilized Recombinant Mouse CD40 (C-Fc) (orb2994384) at 2μg/ml (100 μl/well) can bind Recombinant Mouse CD40L (N-6His) (orb2994091).The EC50 of Recombinant Mouse CD40L (N-6His) (orb2994091) is 12.91ng/ml.(Regularly tested)
• Tag: N-6His
• Molecular Weight: Predicted: 18.8 KDa. Observed: 20 KDa, reducing conditions
• Expression Region: Met112-Leu260
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD40 Ligand; CD40LG;HIGM1; T-B cell-activating molecule; T-BAM; TNFSF5; tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5

Quick Database Links

UniProt

P27548