Recombinant Human CD44 antigen (CD44), partial (Active)

• Catalog Number: orb1095907
• Category: Proteins
• Description: Recombinant Human CD44 antigen(CD44),partial (Active)
• Tag: C-terminal hFc-tagged
• Form/Appearance: Lyophilized powder
• Buffer/Preservatives: Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
• Purity: Greater than 90% as determined by SDS-PAGE.
• Protein Sequence: QIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIP
• Protein Length: Partial
• UniProt ID: P16070
• MW: 51.0 kDa
• Application notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Endotoxins: Less than 1.0 EU/ug as determined by LAL method.
• Source: Mammalian cell
• Biological Origin: Homo sapiens (Human)
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized CD44 at 2 μg/ml can bind Anti-CD44 mouse monoclonal antibody, the EC50 of the CD44 protein is 11.89-14.94 ng/ml.
• Expression Region: 21-220aa
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
• Alternative names: CDw44 (Epican) (Extracellular matrix receptor III)
• Research Area: Cancer Biology
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) CD44.

Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) CD44.

Measured by its binding ability in a functional ELISA. Immobilized CD44 at 2 μg/ml can bind Anti-CD44 mouse monoclonal antibody, the EC50 of the CD44 protein is 11.89-14.94 ng/ml.

The purity of CD44 was greater than 95% as determined by SEC-HPLC