Rat IgG (H&L) Antibody Alkaline Phosphatase Conjugated

• Catalog Number: orb347903
• Category: Antibodies
• Description: Rat IgG (H&L) antibody (Alkaline Phosphatase)
• Clonality: Polyclonal
• Species/Host: Gallus
• Isotype: IgG
• Conjugation: AP
• Reactivity: Rat
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.2 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Chicken Serum, Rat IgG and Rat Serum.
• Immunogen: Rat IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application notes: Anti-Rat IgG Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 µg of Rat IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,400 to 1:6,100 of the stated concentration is suggested for this product.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Alternative names: Chicken Anti-Rat IgG alkaline phosphatase Conjugat
• Note: For research use only

Western Blot of Anti-Rat IgG (H&L) (CHICKEN) Antibody (p/n orb347900). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n orb2652744). Lane 2: Rat IgG F(c) Fragment (p/n orb346338). Lane 3: Rat IgG Fab Fragment (p/n orb346340). Lane 4: Rat IgM Whole Molecule (p/n orb346341). Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (CHICKEN) Antibody (p/n orb347900) 1:1000 for 60 min at RT. Secondary Antibody: Anti-Chicken IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb346892) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.