Rat IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

• Catalog Number: orb347867
• Category: Antibodies
• Description: Rat IgG (H&L) antibody (Alkaline Phosphatase)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: AP
• Reactivity: Rat
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against Human Serum Proteins.
• Immunogen: Rat IgG whole molecule
• Tested applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:1,500 - 1:7,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
• Application notes: Anti-Rat IgG Alkaline Phosphatase antibody has been tested by ELISA and dot blot and is suitable for use in immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Alternative names: Rabbit Anti-Rat IgG alkaline phosphatase Conjugate
• Note: For research use only

Dot Blot results of Rabbit Anti-Rat IgG Antibody Alkaline Phosphatase Conjugate. Dots are Rat IgG: (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Primary Antibody: none. Secondary Antibody: Rabbit Anti-Rat IgG Antibody Alkaline Phosphatase at 1 ug/ml in orb348637 1hr RT.