SOX10 Rabbit Recombinant Monoclonal Antibody

SKU: orb1519802

Description

SOX10 Antibody

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Dilution Range: (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 20 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
• Reactivity: Human, Mouse
• Application Notes:
  All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BLR080G
• Immunogen: Between 416 and C-terminus
• Target: SOX10
• Purity: Recombinant antibody was purified from cell culture supernatant
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 50 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DOM; dominant megacolon, mouse, human homolog of; PCWH; SRY (sex determining region Y)-box 10; SRY-related HMG-box gene 10; transcription factor SOX-10; WS2E; WS4; WS4C

Detection of human SOX10 in FFPE SK-MEL-28 cells by ICC. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody (orb1519802). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human SOX10 by western blot. Samples: Whole cell lysate (15 µg) from HEK293T, Malme-3M, MCF-7, SK-MEL-28, Jurkat, MDA-MB-435, HeLa, and UACC-62 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-GAPDH

Detection of human SOX10 in FFPE melanoma by IHC. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB

Detection of human SOX10 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB

Quick Database Links

Gene Symbol

SOX10

UniProt

P56693

RefSeq (Protein):NP_008872.1