Rabbit anti-Lamin-A/C Antibody

• Catalog Number: orb1524853
• Category: Antibodies
• Description: Lamin-A/C Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 1 and 50
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:100 - 1:500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Lamin-A/C
• UniProt ID: P02545
• NCBI: NP_733821.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: prelamin-A/C; LDP1; LGMD1B; LMN1; LMNC; LMNL1; lam
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Lamin-A and Lamin-C by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Lamin-A/C antibody orb1524853 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. Lamin-A was also immunoprecipitated by rabbit anti-Lamin-A antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).

Detection of mouse Lamin-A /C by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Lamin-A/C (Cat. No. orb1524853) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human Lamin-A /C by immunohistochemistry. Samples: FFPE sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-Lamin-A/C (Cat. No. orb1524853) used at a dilution of 1: 200 (1µg/ml) (left) and 1: 1, 000 (0.2µg/ml) (right). Detection: DAB

Detection of human Lamin-A /C by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Lamin-A/C (Cat. No. orb1524853) used at a dilution of 1: 200 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100.