Rabbit anti-Lamin-A Antibody

• Catalog Number: orb1524849
• Category: Antibodies
• Description: Lamin-A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: Between 425 and 475
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:50 - 1:500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Lamin-A
• UniProt ID: P02545
• NCBI: NP_733821.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: prelamin-A/C; LDP1; LGMD1B; LMN1; LMNC; LMNL1; lam
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Lamin-A by western blot. Samples: Whole cell lysate (15 and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-Lamin-A antibody orb1524849 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Lamin-A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Lamin-A antibody orb1524849 used for IP at 6 µg/mg lysate.

Detection of human Lamin-A by immunohistochemistry.Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Lamin-A (Cat. No. orb1524849) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of mouse Lamin-A by immunohistochemistry.Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-Lamin-A (Cat. No. orb1524849) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human Lamin-A by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Lamin-A (Cat. No. orb1524849) used at a dilution of 1: 200 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100.