Rabbit KLF4 Recombinant Monoclonal Antibody

SKU: orb1710839

Description

KLF4 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ICC, IHC, IP, WB
• Dilution Range: WB: 1:1,000, IP: 6 µl/1 mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BLR250L
• Immunogen: Between 75 and 125
• Target: KLF4
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

endothelial Kruppel-like zinc finger protein; epithelial zinc finger protein EZF; EZF; GKLF; gut Kruppel-like factor; gut-enriched krueppel-like factor; Krueppel-like factor 4; Kruppel-like factor 4 (gut)

Detection of human KLF4 by western blot. Samples: Whole cell lysate (25 µg) from DLD-1, PC-3, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse KLF4 by western blot. Samples: Whole cell lysate (25 µg) from CH27, CT26, C2C12, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse KLF4 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human KLF4 by immunohistochemistry. Sample: FFPE section of human gastric carcinoma. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human KLF4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839) used for IP at 6 µl/mg lysate.

Detection of human KLF4 by immunocytochemistry. Sample: FFPE section of HCT 116 cells. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse KLF4 by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

KLF4

UniProt

O43474

RefSeq (Protein):NP_004226.3