Rabbit anti-FOXP3 Recombinant Monoclonal Antibody

• Catalog Number: orb1519916
• Category: Antibodies
• Description: FOXP3 Antibody
• Target: FOXP3
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Purity: Recombinant antibody was purified from cell culture supernatant
• Immunogen: Between 275 and 325
• UniProt ID: Q9BZS1
• Tested applications: ICC, IF, IHC, IP, WB
• Dilution range: Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 20 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
• Application notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
• Antibody Type: Primary Antibody
• Clone Number: BLR034F
• Storage: 2 - 8°C
• Alternative names: PIDX; scurfin; JM2; IPEX; immunodeficiency, polyen
• Background: FOXP3 is a transcriptional regulator which is cruc
• Note: For research use only
• NCBI: NP_054728.2
• Expiration Date: 12 months from date of receipt.

Detection of human FOXP3 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, MJ, MOLT-4, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody

Detection of human FOXP3 in FFPE tonsil by immunohistochemistry. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human FOXP3 in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB

Detection of human FOXP3 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue)