FOXP3 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb559206
• Category: Antibodies
• Description: FOXP3 Recombinant Rabbit Monoclonal Antibody
• Target: FoxP3
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: Recombinant human FOXP3 protein, around C-terminal 150aa
• UniProt ID: Q9BZS1
• MW: 47 kDa
• Tested applications: FC, ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-100
• Antibody Type: Primary Antibody
• Clone Number: 12B1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Forkhead box protein P3; forkhead box P3; Scurfin;
• Research Area: Cell Biology, Immunology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: 293T (Mouse) Lysate at 40 ug, Primary: Anti-FOXP3 (orb559206) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 47 kD.

Blank control: MCF-7. Primary Antibody (green line): Rabbit Anti-FOXP3 antibody (orb559206), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-FOXP3 antibody (orb559206), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.