Rabbit anti-ARID1A/BAF250 Recombinant Monoclonal Antibody

SKU: orb1806460

Description

Rabbit anti-ARID1A/BAF250 Recombinant Monoclonal Antibody

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution range: WB: 1:1000 (3-8% Tris-acetate gel recommended), IP: 20 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Clone No.: BLR279L
• Immunogen: Between 750 and 800
• Target: ARID1A/BAF250

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 50 µg/ml
• Disclaimer: For research use only

Alternative Names

osa homolog 1; SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1; hOSA1; hELD; MRD14; OSA1; OSA1 nuclear protein; P270; ELD; SWI/SNF complex protein p270; BRG1-associated factor 250; SWI-like protein; SMARCF1; B120; ARID domain-containing protein 1A; C1orf4; AT-rich interactive domain-containing protein 1A; CSS2; BAF250; BAF250a; BM029; brain protein 120; BRG1-associated factor 250a; chromatin remodeling factor p250; AT rich interactive domain 1A (SWI-like)

Detection of human ARID1A/BAF250 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, RKO, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (orb1806460) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse ARID1A/BAF250 by western blot. Samples: Whole cell lysate (50 µg) from F9, NIH 3T3, A20, CH27, and ND7/23 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (orb1806460) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse ARID1A/BAF250 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human ARID1A/BAF250 by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse ARID1A/BAF250 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti- ARID1A recombinant monoclonal antibody (orb1806460) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human ARID1A/BAF250 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human ARID1A/BAF250 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (orb1806460) used for IP at 20 µl/mg lysate.

Detection of mouse ARID1A/BAF250 by immunocytochemistry. Sample: FFPE section of mouse mIMCD-3 cells. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human ARID1A/BAF250 by immunocytochemistry. Sample: FFPE section of OVCAR-8 cells. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

ARID1A/BAF250

UniProt

O14497