Rabbit anti-Alpha-Adducin Antibody

SKU: orb1524323

Description

Alpha-Adducin Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 650 and 700
• Target: Alpha-Adducin
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

ADDA; Erythrocyte adducin subunit alpha; erythrocyte adducin alpha subunit; adducin 1 (alpha); alpha-adducin

Detection of human Alpha-Adducin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Alpha-Adducin antibody orb1524323 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human Alpha-Adducin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Alpha-Adducin antibody orb1524323 used for IP at 6 µg per reaction.

Detection of human Alpha-Adducin by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Alpha-Adducin (Cat. No. orb1524323) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human Alpha-Adducin by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Alpha-Adducin Cat. No. orb1524323 used at a dilution of 1: 5, 000 (0.2µg/ml) (right) and Cat. No. orb1524325 used at a dilution of 1: 5, 000 (0.2µg/ml) (left). Detection: DAB

Quick Database Links

Gene Symbol

Alpha-Adducin

UniProt

P35611

RefSeq (Protein):NP_001110.2