PTK2 Antibody (Center)

SKU: orb1928396

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD42997
• Immunogen: This PTK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-423 amino acids from the Central region of human PTK2. Antigen Region: 396-423 aa.
• Target: PTK2 (HGNC:9611)
• Molecular Weight: 119233 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, FRNK, Protein phosphatase 1 regulatory subunit 71, PPP1R71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, PTK2, FAK, FAK1

Western blot analysis of PTK2 Antibody (Center) in T47D cell line lysates (35 ug/lane). PTK2 (arrow) was detected using the purified Pab.

Flow cytometric analysis of K562 cells using PTK2 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of PTK2 (arrow) using rabbit polyclonal PTK2 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK2 gene (Lane 2).

Confocal immunofluorescent analysis of PTK2 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human brain tissue reacted with PTK2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

PTK2 (HGNC:9611)

UniProt

Q05397

RefSeq (Protein):NP_001186578.1, NP_722560.1