Polyinosinic-polycytidylic acid

SKU: orb1305706

Description

Poly(I:C) is a synthetic dsRNA analog that activates TLR3, RIG-I, and MDA5 pathways, eliciting robust innate immune responses. It is widely used as a vaccine adjuvant and in immunology research, including the establishment of ARDS animal models and in vitro studies of antiviral and antitumor immunity.

Research Area

Cell Biology, Immunology & Inflammation, Metabolism Research, Signal Transduction

Key Properties

• CAS Number: 24939-03-5
• MW: 671.406
• Purity: 90.00%
• Formula: (C₁₀H₁₃N₄O₈P)x.(C₉H₁₄N₃O₈P)x
• SMILES: Nc1ccn([C@@H]2O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]2O)c(=O)n1.O[C@@H]1[C@@H](COP(O)(O)=O)O[C@H]([C@@H]1O)n1cnc2c1nc[nH]c2=O
• Target: Interleukin,Serine/threonin kinase,HSP,Bcl-2 Family,Apoptosis,TLR
• Solubility: DMSO:< 1 mg/mL (insoluble or slightly soluble);H2O:128.8 mg/mL

Bioactivity

• In Vivo:
  METHODS: To investigate the neuroprotective effects in an acute ischemia model, Polyinosinic-polycytidylic acid (0.3 mg/kg) was administered as a single intramuscular injection to Kun-Ming strain mice, and a model of arterial occlusion (MCAO) was constructed 2 h later. RESULTS: Administration of polyinosinic-polycytidylic acid significantly attenuated neurological deficits in the ischemic striatum and cortex, reduced infarct volume, and suppressed the elevation of TNFα and IL-6 levels. METHODS: To test the antitumor activity in vivo, Polyinosinic-polycytidylic acid (1-100 μg/mouse) was intraperitoneally injected into a mouse model of metastatic tumors C57BL/6J induced by cutaneous melanoma B16-F10. RESULTS: Lung tumor growth stopped after a single dose of Polyinosinic-polycytidylic acid. Polyinosinic-polycytidylic acid has potential antitumor activity in an established mouse model of lung metastasis.
• In Vitro:
  METHODS: Cervical cancer cells HeLa, SiHa, C33A and lung cancer cells A549 were treated with polyinosinic-polycytidylic acid (0.1-1 μg/mL) for 24 h, and cell death was detected using PI Staining. RESULTS: Polyinosinic-polycytidylic acid effectively induced tumor cell death in a dose-dependent pattern. METHODS: Rat astrocytes were pretreated with polyinosinic-polycytidylic acid (10-20 μg/mL) for 12 h, and then exposed to oxygen-glucose deprivation (OGD) for 12 h. The morphology of the cells was examined by microscopy. RESULTS: OGD induced significant cellular damage, and the cells appeared to be healthier in the group pretreated with polyinosinic-polycytidylic acid.Polyinosinic-polycytidylic acid exerted a certain degree of protective effect against OGD-induced damage in cultured astrocytes.

Storage & Handling

• Storage: store at low temperature,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, Polyinosinic-polycytidylic acid, Polyinosinic polycytidylic acid, Polyinosinicpolycytidylic acid, Poly(I:C), Toll-like Receptor (TLR), TLR3

Compound Identifiers

PubChem CID

135618150