PI3KC3 Antibody (N-term)

SKU: orb38852

Description

Purified rabbit polyclonal antibody.

Research Area

Cell Biology, Immunology & Inflammation, Immunology & inflammation, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, IHC-P - 1:50-100
• Reactivity: Human
• Predicted Reactivity: Mouse, Other, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD3817
• Immunogen: Synthetic Peptide
• Target: PIK3C3
• Molecular Weight: 101549
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Phosphatidylinositol 3-kinase catalytic subunit type 3 antibody, anti-PtdIns-3-kinase type 3 antibody, anti-PI3-kinase type 3 antibody, anti-PI3K type 3 antibody, anti-Phosphoinositide-3-kinase class 3 antibody, anti-Phosphatidylinositol 3-kinase p100 subunit antibody, anti-vps34 antibody, anti-PI 3 Kinase Class 3 antibody

Confocal immunofluorescent analysis of PI3KC3 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of lysates from Hela, SH-SY5Y cell line (from left to right), using PI3KC3 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

PIK3C3

UniProt

Q8NEB9

RefSeq (Protein):NP_002638.2