Phospho-RAD9(S328) Antibody

SKU: orb34504

Description

Peptide affinity purified rabbit polyclonal antibody.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: DOT, IHC-P, WB
• Dilution Range: DOT: 1:500, WB: 1:1000, IHC-P: 1:50-100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD9337
• Immunogen: Synthetic Peptide
• Target: RAD9A
• Molecular Weight: 42547
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Cell cycle checkpoint control protein antibody, anti-RAD9 homolog A antibody, anti-S. pombe antibody, anti-RAD9A antibody

The anti-Phospho-Rad9-S328 Pab is used in Western blot to detect Phospho-Rad9-S328 in HL60 (left) and Y79 (right) tissue lysates.

Dot blot analysis of anti-Phospho-Rad9-S328 Antibody on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

RAD9A

UniProt

Q99638

RefSeq (Protein):NP_004575.1, NP_001230153.1

Huang, Ting-Hsiang et al. The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells Mol Cell, 69, 879-892.e5 (2018)

PubMed: 29478807