Phospho-PPAR Gamma (ser273) Rabbit Polyclonal Antibody

• Catalog Number: orb158188
• Category: Antibodies
• Description: Phospho-PPAR Gamma (ser273) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Gallus, Mouse, Rabbit, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human PPAR Gamma around the phosphorylation site of ser273 DK(p-S)PF
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 57 kDa
• Target: PPARG
• UniProt ID: P37231
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: phospho-PPAR gamma(ser273); P-PPAR gamma(phospho-s
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Marfella, Raffaele et al. Sodium/glucose cotransporter 2 (SGLT2) inhibitors improve cardiac function by reducing JunD expression in human diabetic hearts Metabolism, 127, 154936 (2021)

PubMed: 34801581

phosphopeptide, non phosphopeptide.

Sample: Lane 1: Human A549 cell lysates, Lane 2: Human 293T cell lysates, Lane 3: Human HepG2 cell lysates, Primary: Anti-Phospho-PPAR Gamma (ser273) (orb158188) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kDa, Observed band size: 57 kDa.

Tissue/Cell: A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-PPAR Gamma (ser273)) polyclonal Antibody, Unconjugated (orb158188) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: human gastric carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-PPAR Gamma (ser273) Polyclonal Antibody, Unconjugated (orb158188) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-PPAR Gamma (ser273) Polyclonal Antibody, Unconjugated (orb158188) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.