Phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) Rabbit Polyclonal Antibody

• Catalog Number: orb106105
• Category: Antibodies
• Description: Phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PI3 kinase p85 alpha around the phosphorylation site of Tyr467 RL(p-Y)EE
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 85/55 kDa
• Target: PIK3R1
• UniProt ID: P27986
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PI3 kinase p85 alpha(phospho Tyr467); PI3 kinase p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Chen, Yanbo et al. Pretreatment of ghrelin protects H9c2 cells against hypoxia/reoxygenation-induced cell death via PI3K/AKT and AMPK pathways Artif Cells Nanomed Biotechnol, 47, 2179-2187 (2019)

PubMed: 31159591

Deng, Dan et al. Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21 Artif Cells Nanomed Biotechnol, 48, 610-619 (2020)

PubMed: 32050796

Wang, Baoying et al. Puerarin attenuates hypoxia-resulted damages in neural stem cells by up-regulating microRNA-214 Artif Cells Nanomed Biotechnol, 47, 2746-2753 (2019)

PubMed: 31282213

Zhang, Kexiang et al. MicroRNA-211/BDNF axis regulates LPS-induced proliferation of normal human astrocyte through PI3K/AKT pathway Biosci Rep, 37, (2017)

PubMed: 28790168

Wang, Yanan et al. Luteolin Partially Inhibits LFA-1 Expression in Neutrophils Through the ERK Pathway Inflammation, 42, 365-374 (2019)

PubMed: 30255285

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199)/FITC Conjugated antibody, Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-PI3 kinase p85 alpha+gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated (orb106105) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.