Phospho-P21CIP1(T145) Antibody

SKU: orb1931249

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: DOT, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100, DB - 1:500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD29734
• Immunogen: This P21CIP1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T145 of human P21CIP1.
• Target: CDKN1A (HGNC:1784)
• Molecular Weight: 18119 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1

Dot blot analysis of anti-Phospho-P21CIP1-T145 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The anti-Phospho-P21CIP1-T145 Pab is used in Western blot to detect Phospho-P21CIP1-T145 in Hela tissue lysate.

Quick Database Links

Gene Symbol

CDKN1A (HGNC:1784)

UniProt

P38936

RefSeq (Protein):NP_000380.1, NP_001207707.1, NP_001207706.1, NP_510867.1