Phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) Rabbit Polyclonal Antibody

SKU: orb185140

Description

Phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) Rabbit Polyclonal Antibody

Research Area

MAPK/ERK Signalling Pathway, Oxidative Stress

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Equine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human MST4/MST3/STK25 around the phosphorylation site of Thr178/Thr190/Thr174 RN(p-T)FV
• Target: STK25
• Molecular Weight: 48 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

phospho-MST4+MST3+STK25(phospho T178+T190+T174); p-MST4+MST3+STK25(phospho T178+T190+T174); Mammalian STE20 like protein kinase 3; Mammalian Ste20 like protein kinase 4; Mammalian sterile 20 like 4; MASK; mess1; MGC94619; MST 3; MST 4; MST; Mst2; MST3 and SOK1 related kinase; MST3; MST3B; OTTHUMP00000018592; OTTHUMP00000018593; Protein kinase homolog serine/threonine kinase 3(Ste20 yeast homolog); RGD1563568; RP11-111L24.5; Serine/threonine kinase 24(STE20 homolog yeast); Serine/threonine kinase 24; Serine/threonine kinase 25; Serine/threonine protein kinase 24; Serine/threonine protein kinase MASK; Serine/threonine protein kinase MST 4; Serine/threonine protein kinase MST4; SOK1; STE20; Ste20 like; Ste20 like kinase; STE20 like kinase 3; STE20 like kinase MST 4; STE20 like kinase MST3; STE20 like kinase MST4; Ste20 yeast homolog; Ste20/oxidant stress response kinase 1; Sterile 20(oxidant stress response kinase 1); sterile 20 like kinase 3; Sterile 20/oxidant stress-response kinase 1; STK3; Yeast Sps1/Ste20 related kinase 1; YSK1.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174)) Polyclonal Antibody, Unconjugated (orb185140) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174)) Polyclonal Antibody, Unconjugated (orb185140) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174)) Polyclonal Antibody, Unconjugated (orb185140) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174)) Polyclonal Antibody, Unconjugated (orb185140) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174)) Polyclonal Antibody, Unconjugated (orb185140) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: U-87MG (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) (orb185140) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 39 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-MST4+MST3+STK25 (Thr178+Thr190+Thr174) Polyclonal Antibody, Unconjugated (orb185140) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

STK25

UniProt

O00506