Phospho-LATS1+LATS2 (Thr1079 +Thr1041) Rabbit Polyclonal Antibody

• Catalog Number: orb100648
• Category: Antibodies
• Description: Phospho-LATS1+LATS2 (Thr1079 +Thr1041) Rabbit Polyclonal Antibody
• Target: LATS1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rabbit
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human LATS1 around the phosphorylation site of Thr1079 EF(P-T)FR
• UniProt ID: Q9NRM7
• MW: 124 kDa
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: LATS1+LATS2(phospho T1079+T1041); Serine/threonine
• Research Area: CDK Regulators, Cancer Research, Cell Biology, Cel
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-LATS1+LATS2 (Thr1079 +Thr1041)) Polyclonal Antibody, Unconjugated (orb100648) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

phosphopeptide, non phosphopeptide.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) (orb100648) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 124 kD, Observed band size: 124 kD.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) (orb100648) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 124 kD, Observed band size: 124 kD.