Phospho-IRAK1 (Thr387) Rabbit Polyclonal Antibody

• Catalog Number: orb6223
• Category: Antibodies
• Description: Phospho-IRAK1 (Thr387) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IRAK1 around the phosphorylation site of Thr387 RG(p-T)LA
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg /Test, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 78 kDa
• Target: Irak1
• UniProt ID: P51617
• RRID: AB_10936318
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: IRAK(phospho T387); p-IRAK(phospho T387); IRAK1(ph
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Summers, Lauren et al. Activation of brain endothelial cells by interleukin-1 is regulated by the extracellular matrix after acute brain injury Mol Cell Neurosci, 57, 93-103 (2013)

PubMed: 24161715

Blank control (blue): RSC96 cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospho-IRAK1 (Thr387) antibody (orb6223), dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IRAK1 (Thr387)) Polyclonal Antibody, Unconjugated (orb6223) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-IRAK1 (Thr387)) Polyclonal Antibody, Unconjugated (orb6223) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IRAK1 (Thr387)) Polyclonal Antibody, Unconjugated (orb6223) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

phosphopeptide, non phosphopeptide.

Sample: Lane 1: Liver (Mouse) Cell Lysate at 30 ug, Lane 2: Colon carcinoma (Human) Lysate at 30 ug, Primary: Anti-Phospho-IRAK1 (Thr387) (orb6223) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 70kD, Observed band size: 70kD.

Tissue/Cell: mouse spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-IRAK1 (Thr387) Polyclonal Antibody, Unconjugated (orb6223) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.